Malignant cells in ascitic fluid cytology

Ascitic fluid cytology of a malignant mixed Müllerian

In approximately 50% of cases, the presence of ascites in patients with known neoplasms heralds the presence of peritoneal carcinomatosis. 22,23 In these patients, malignant cells can be detected in the fluid up to 97% of the time, making this test very sensitive and the gold standard for diagnosing peritoneal carcinomatosis. 22 In contrast, positive cytology findings are variable in patients with hepatic metastases, lymphoma, or hepatocellular carcinoma Here, we report ascitic fluid cytology for two peritoneal MMMT cases. Immunocytochemistry for p53 was helpful in identifying malignant cells; tumor cells in ascitic fluid smears, as well as carcinomatous cells and sarcomatous cells in tissue sections, showed distinct nuclear immunostaining for p53, whereas mesothelial cells did not

(Pdf) Ascitic Fluid Cytology in Suspected Malignant

Positive ascitic fluid cytology is needed to establish the diagnosis of malignancy-related ascites, if this is in doubt e.g. a patient with a history of cirrhosis. The overall sensitivity of cytology for the detection of malignancy-related ascites is 58-75%. Send ascitic fluid for investigation to help confirm diagnosis and exclude infection Out of the 48 ascitic fluid samples cytological diagnosis of benign effusions was rendered in (76%) cases and suspicious for malignancy in (16%) cases where as in cellblock malignant effusions were diagnosed in (32%) cases. There was no diagnosis of suspicious for malignancy in cellblock. (Table 1) Ascitic fluid cell count and differential Polymorphonuclear leukocyte (PMN) count > 250 cells/mm 3 in the absence of an intra abdominal, surgically treatable source of infection indicates spontaneous bacterial peritonitis ( Hepatology 1982;2:399 ), which complicates about 12% of cirrhotic patients with ascites ( Dig Liver Dis 2001;33:41 Of these 276 ascitic cytology specimens 48 (17%) were found to contain malignant cells. Malignant cells that were ovarian in origin were found in 41 (85%) patients of the positive samples analysed. The remaining positive specimens showed that three patients had lymphoma, one breast metastasis, one carcinoid, and two had an unknown primary

Ascites Fluid Cytology - an overview ScienceDirect Topic

Klebs were apparently the first investigators who recognized the presence of malignant cells in an ascitic fluid in 1867. In 1882 Quincke was credited for detailed descriptions of ovarian and lung cancer cells in serous effusions. Since that time reports on effusio diagnostic yield for malignancy. In ascitic fluid, out of 25 Out of the 25 ascitic fluid samples cytological diagnosis of benign effusions was rendered in 19 (76%) cases and suspicious for malignancy in 4(16%) cases in conventional smear where as in cellblock malignant effusions we diagnosed in 8 (32%) cases. study. Kappa v Fluid cytology has a clinical significance in the management of patients with malignancy. Effusions are often the first clinical symptom of malignant tumors or of their metastatic manifestation. In known malignancies effusins are an omnious sign. In one third of malignant effusions, cytology gives the first indication of malignancy Malignant ascites can develop through several mechanisms: blocked lymphatic channels as a result of malignancy, direct production of fluid into the peritoneal cavity by highly active cancers, and.. Fractal dimensions of reactive mesothelial cells and malignant cells in ascitic fluid cytology. Bhatia A, Eckka RS, Dey P. PMID: 20701104 [PubMed - indexed for MEDLINE] Publication Types: Letter; MeSH Terms. Adenocarcinoma/diagnosis* Adenocarcinoma/secondary; Ascitic Fluid/pathology* Cell Nucleus/pathology; Cell Proliferation; Epithelium/pathology* Fractals* Human

SERUM-TO-ASCITES ALBUMIN GRADIENT (SAAG) =Serum albumin - ascitic fluid albumin If the gradient is >1.1: Portal HTN (drives fluids into peritoneum) SBP, cirrhosis, Alcoholic hepatitis, CHF If the gradient is < 1.1: (protein leaks into peritoneum and fluid follows) Peritoneal carcinomatosis, peritoneal TB, pancreatitis, nephrotic syndrom Introduction. Cytology specimens for diagnostic purposes can be obtained by exfoliation or aspiration of cells from cancer patients. Ascites and peritoneal washing fluids are common sources of cell specimens from gastrointestinal (GI) cancer patients (1, 2).Ascites, defined as the abnormal collection of fluid in the peritoneal cavity, can occur due to cancers and other diseases, including. • Distinguish benign from malignancy cytology on CSF and body fluids • Recommend appropriate further evaluation when necessary 2. Malignant Ascitic Fluid Male Misc 38 - 46.4% GI tract 23.8 - 42.8% Unknown 14.2 - 16.6% Lung 4.7 - 9.5% • Vacuoles in malignant cells usually represent synthesized material (e.g. mucin

Malignant Ascites - an overview ScienceDirect Topic

  1. ation is most successful in patients with ascites related to peritoneal carcinomatosis as viable malignant cells are exfoliated into the ascitic fluid. However, only approximately 53% of patients with malignancy-related ascites have peritoneal carcinomatosis
  2. Mesothelial cytopathology is a large part of cytopathology. The article deals with cytopathology specimens from spaces lined with mesothelium, i.e. it deals with pericardial fluid, peritoneal fluid and pleural fluid. An introduction to cytopathology is in the cytopathology article
  3. al fluid, peri-hepatic. Image: cytological speciemen: Pleural fluid. Cytological Report: Presence of many epithelial malignant tumor cells isolated, with eccentric nucleus and large cytoplasmic vacuoles, mixed with rare mesothelial cells, some lymphocytes and granulocytes. Positive for malignancy
  4. , protein, cytology, and cultures. These studies can be used to evaluate the patient for both infectious and.

Cell block technique was first described by Bahrenberg in 1896. This is an old method for evaluation of body cavity fluids. The cell block technique employs the retrieval of cells or small tissue fragments from any body fluid including ascitic fluid, pleural fluid, bronchial wash and imaging guided fine needle aspiration cytology specimens Malignant cells were seen in 28.7% of all the samples, while 51.2% were negative for malignant cells. Malignant cells were seen in 4 (6.7%), 11 (18.3%) and 6 (10%) of the AFC performed due to various liver pathologies, ovarian malignancies and intra-abdominal malignancies respectively. Malignant cells were found more in females with a male to. fluid production by tumor cells lining peritoneal cavity in cases of peritoneal carcinomatosis (PC), alteration in vascular Ascitic fluid analysis is considered the gold standard investigation [4]. Tumor markers are not diagnostic for malignancy but may ascites with positive malignant cytology, hemorrhagic or chylous ascites an A critical evaluation of 3000 cytologic analyses of pleural fluid, ascitic fluid, and pericardial fluid. Acta Cyto 1966; 10:455. Decker D, Stratmann H, Springer W, et al. Benign and malignant cells in effusions: diagnostic value of image DNA cytometry in comparison to cytological analysis tive value (NPV) of ascites fluid in diagnosis of malignancy were calculated. Further analysis was done to evaluate the performance of IHC of cell blocks of ascites fluid in diagnos-ing malignancy of ovarian origin. RESULTS Between 2010 and 2015, 551 patients older than 51 years had both cytology and diagnostic histology samples taken BARANSI ET A

Malignant ascites also show more lymphocytes. RBCs presence indicates malignancies, tuberculosis, or intra-abdominal bleeding. WBCs presence indicates peritonitis, tuberculosis, or cirrhosis. Cytology: This can be done better on cytospin and can differentiate the cells and as well find the malignant cells CT images of a patient on initial recurrence of malignant ascites without other CT findings related to peritoneal seeding, later confirmed by cytology. A small volume of pelvic ascites fluid was observed at first appearance (A). Follow-up CT (B) showed an increased volume of ascites fluid. Yellow stars indicate ascites

Ascitic fluid cytology of a malignant mixed müllerian

Lymphocytes: CHF, liver cirrhosis, nephrotic syndrome Mesothelial Cells: Associated with TB effusions Malignant cells: seen with malignancy 44. WHAT TO SEND FLUID FOR Cell count with diff Albumin LDH Total protein glucose Gram stain/cx cytology 45 CHARACTERISTICS OF MALIGNANT ASCITES Malignant ascites is characterized by positive cytology of malignant cells, large number of white blood cells and a higher lactate dehydrogenase level (14, 21). Interestingly, the main ascitic fluid protein-levels are high in patients with peritonitis carcinomatosa, as are ascites albumin concentrations (21) Evaluation combined with findings of ascites cells and CT scan suggested that the elevated serum CA-125 was related to PL. Ascites cytology is important to diagnose malignant ascites. However, the sensitivity rate is affected by the number and quality of specimens processed PDF | On Oct 1, 2009, Alka Bhatia and others published Fractal dimensions of reactive mesothelial cells and malignant cells in ascitic fluid cytology | Find, read and cite all the research you. In the discontinuous Ficoll gradient, concentration of malignant cells at a specific interface was mainly a function of cell density. Thus, while most malignant cells banded in the last two fractions, adenocarcinomas, especially those high in mucus (which decreases total cell density) banded higher in the gradient

Cytology description. Staging incorporates presence of malignant cells in washings or ascites fluid Serous / endometrioid carcinomas more often have positive cytology than other carcinomas, particularly if high grade Cytology images. AFIP images. Ovarian carcinoma cells in cervical aspirate While mean ascitic fluid protein concentration showed a significant difference (P<0.01) between the two groups, there was no difference in respect to ascitic fluid pH, glucose concentration and leucocyte count. Malignant cell cytology was positive in 54.5% of group A patients with no false positive report in group B

Malignant ascites is a formation of fluids and cancer cells in the abdominal cavity. Patients who suffer from this are usually on the final stage of their cancer events. According to the most recent study conducted by a team of oncologists, this is a common occurrence for people with ovarian and gastrointestinal cancer cinomatosis had positive ascitic fluid cytology. Ascitic fluid analysis is helpful in identifying and distinguishing the subgroups of malignancy-related ascites. Malignancies that originate in or metastasize to the abdomen can cause ascites formation by several mecha- nisms. Fluid can exude from malignant cells lining the peritoneum. Shivakumarswamy Udasimath et al., Role of the Cell Block Method in the Diagnosis of Malignant Ascitic Fluid Effusions www.jcdr.net Journal of Clinical and Diagnostic Research, 2012 September.

Malignant-related ascites - GGC Medicine

Background: Multiple myeloma or plasmacytoma resulting in malignant effusion is rarely described in literature. Aims: In this paper, we have studied the seven rare cases of plasma cell infiltration in effusion fluid. Materials and Methods: We studied six cases of pleural fluid and one case of ascetic fluid. Detailed cytological features, clinical history, bone marrow examinations, serum. The presence of malignant cells on ascitic fluid smear cytology may be the first indication of malignancy in around half of all patients with peritoneal carcinomatosis and is generally regarded as a poor prognostic sign.[10,11,12,15] Malignancies of the FGT, primarily from the ovary in 12 cases, and peritoneal carcinomatosis (9) with primaries. On inspection, most ascitic fluids are transparent and tinged yellow. In the case of malignancy, it could also appear pink or red (when at least 10,000 red blood cells/μL are present). Any inflammatory condition can cause an elevated white blood cell count. In case of malignant ascites, lymphocytes usually predominate

A minimum of 10,000 red blood cells/µL is required for ascitic fluid to appear pink, and more than 20,000 red blood cells/µL will produce distinctly blood-tinged fluid. This may result from either a traumatic tap or malignancy. Bloody fluid from a traumatic tap is heterogeneously bloody, and the fluid will clot The malignant cells in the pleural or the ascitic fluids were almost always indicative of metastatic tumours, as primary malignancies which arose from the mesothelial cell lining were uncommon. A positive effusion for malignant cells is an important prognostic indicator in cancer patients . The development of a malignant pleural effusion is a. In this study, routine conventional smears and cell block from ascitic fluid were studied for cellularity, cytological preservation of architecture and its diagnostic utility and grading of malignancy. The objective of this study is to evaluate the utility of routine use of cell block to assess the concordance in diagnosis between conventional. Alveolar rhabdomyosarcoma is an uncommon tumor affecting adolescents and young adults that is only rarely encountered in body fluid cytology. We report the cytological features of metastatic alveolar rhabdomyosarcoma in the ascitic fluid of a 17-year-old female patient, who had presented with abdominal distention, 21 months after being diagnosed with perirectal alveolar rhabdomyosarcoma The most common site of metastasis to ascitic fluid in females is from a mullerian (ovarian) primary, whereas in males it is from the gastrointestinal tract. Metastatic Merkel cell carcinoma (MCC) to the ascitic fluid is extremely rare and may present as a diagnostic challenge on effusion cytology

Forty-five (17.2%) smears contained malignant cells, 29 (11.1%) were suspicious for malignancy, 108 (41.2%) were negative for malignant cells, and 80 (30.5%) were unsatisfactory. Conclusion: Ascitic fluid cytopathology remains a relevant modality for the prompt evaluation of patients with clinically detectable ascites in our setting A. I. SPRIGGS AND D. W. JERROME From the Laboratory of Clinical Cytology, Churchill Hospital, Oxford and the Department of Histopathology, Radcliffe Infirmary, Oxford SYNOPSIS Three cases of carcinoma of the breast are described, in which similar tumour cells were found in pleural or peritoneal fluid Abstract: Cytological techniques have been universally recognized as the most important diagnostic tool in the recognition of malignant tumours in effusions. To differentiate between reactive mesothelial cells and malignant cells is the diagnostic problem in conventional smear method

Cytology remains the gold standard for the detection of malignant cells in ascites. However, its sensitivity is limited. The aim of this study was to evaluate DNA methylation biomarkers for the differential diagnosis of benign (ascites in patients without malignancy), malignant (ascites in cancer patients directly caused by malignancy), and paramalignant (ascites in cancer patients caused by. Essentials of Fluid Cytology, UBC Pathology, 2009, 2012 Essentials of Gynecologic Cytology, UBC Pathology, 2011 Klebs were apparently the first investigators who recognized the presence of malignant cells in an ascitic fluid in 1867. In 1882 Quincke was credited for detailed descriptions o diagnosis and staging of malignant neoplasms. METHODS: All EUS-guided paracenteses of ascitic fluid per-formed at the University of California Irvine Medical Center (UCIMC) from January 2003 to February 2006 were retrospectively retrieved. Corresponding EUS findings, cytology and histology slides, and follow-up in-formation were reviewed (benign ascites) or a malignant disease without tumor cells in the ascites (paramalignant ascites). Biomarkers allowing for the determination of the prognosis in pa-tients with ascites might be powerful biomarkers for the discrimination between malignant ascites and ascites without tumor cells. Cytology is highly specific for the presence of.

Pathology Outlines - Overview

The results of liver function tests were not available at the time of ascitic fluid cytology. Ascites fluid was sent for cytological examination to the Cyto pathology laboratory. The sample was processed by SurePath TM liquid-based cytology (LBC) technique. The smears showed predominantly a few scattered large malignant cells T-Cell Lymphoma With Raised Serum Glycoprotein-125(CA-125) and Raised Ascitic Fluid Adenosinedeaminase Levels Initially Presenting as Ascites: A Case Report and Review of Literature Chhagan Bihari, M.D.,1 Vikram Bhatia, M.D., D.M.,2 Archana Rastogi, M.D., D.N.B.,1* Vaishali Bhardwaj, M.D., 2 Deepak Jain, F.R.C.R.,3 and Shalini Thapar, D.N.B.3 We report a case of T-cell lymphoma presenting as. Background: Both non-malignant and malignant causes of effusion can be identified by the relatively non-invasive technique of pleural fluid cytology.With this basis the present study on cytology of pleural fluids was taken up. The diagnostic significance of the cytologic study of the fluid may be attributable to the fact that the cell population present in the sediment is representative of a. The results of the cytological test on 179 ascitic fluids and 88 peritoneal washes of patients suspected to have ovarian tumors are critically reviewed to illustrate the difficulties, limitations and future possibilites of this technique. Tissue correlation was available in all cases

While mean ascitic fluid protein concentration showed a significant difference (P < 0.01) between the two groups, there was no difference in respect to ascitic fluid pH, glucose concentration and leucocyte count. Malignant cell cytology was positive in 54.5% of group A patients with no false positive report in group B The basic characteristics of malignant ascites include increased ascitic fluid protein concentration, increase of lactate dehydrogenase, large number of leukocytes, and positive cytology regarding the presence of malignant cells Cytology showed mesothelial cells with reactive changes and no evidence of malignant cells. Biochemical analysis of the ascitic fluid revealed an albumin concentration of 1.7g/dL, total protein 2.5g/dL, lactate dehydrogenase (LDH) 184U/L and amylase 108U/L. Blood parameters were all normal with a CA-125 value of 89u/ml

tomography scan, negative cytology on ascitic fluid analy- sis, and a serum ascites albumin gradient (SAAG) of >ll g/L (1.1 g/dL).' Nine patients had PC. These patients had various diagnoses, but most had adenocarcinoma of un- known primary origin or ovarian carcinoma. PC was con Background Malignant ascites is the accumulation of abdominal fluid due to the direct effects of cancer. This Fast Fact reviews the causes and diagnosis of malignant ascites. Fast Fact #177 will review its treatment. Pathophysiology The pathophysiology of malignant ascites is incompletely understood. Contributing mechanisms include tumor-related obstruction of lymphatic drainage, increased.

Ascitic fluid cytology in suspected malignant effusions

KEY WORDS: ascitic fluid; cytology; pericardial fluid; pleural fluid; sarcoma. INTRODUCTION Sarcomas are uncommon findings in body cavity fluids submitted for cytologic evaluation. They are estimated to represent 3% to 6% of malignant effusions.1 In the largest pediatric series to date, the most commo • many malignant cells overexpress VEGF • high levels have been found in malignant ascites/effusions & in serum of 49-96% of patients with malignant ascites • acts directly on endothelial cells, resulting in macromolecules leaking into the peritoneum, functionally impairing drainage • anti VEGF AB in animal studies decrease ascites 2 ascites and those with malignant cells in the ascitic fluid. Unless the ascites is heavily blood-stained or the patient's anticipated survival is less than 1 month, peritoneovenous shunting is indicated for the treatment of uncontrollable malignant ascites. Cancer 54:2226-2230, 1984. M ALIGNANT ASCITES is a common cause of distres Background Cytological analysis of ascitic fluid is an important tool for diagnosis, staging, and prognostic assessment in patients with cancer, but more detailed information is needed regarding malignancy rates and the time sequence in which ascites develops for different sites of cancer origin. Especially, an increased early tumor diagnosis may improve the acceptance for cytological. The Denver ascites shunt from CareFusion is designed to give you and your patients an alternative to conventional therapy in managing retractable ascites. The Denver ascites shunt is a peritoneo-venous shunting system that can help relieve symptoms of ascitic fluid buildup. The ascites shunt can provide . physiologic benefit

Pathology Outlines - Ascite

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Paracentesis: is the procedure of removing ascitic fluid from the abdominal cavity. The commonest causes of malignant ascites are primary tumours of breast, colon, ovary, stomach, pancreas and bronchus. Paracentesis is a simple procedure, which can be performed as a day case (usually only removing 2-4 litres maximum) ascitic fluid analysis and its diagnostic value, we have , ascitic fibronectin, cholesterol, sialic acid) have been reported to yield a near-perfect discrimination between malignant and nonmalignant ascites, even better than cytologic exami- nation.4- However, in these studies, patients with (cells/mm3) 103 f 102 765 768 NS 1622 f 1066. Fluid cytology in serous cavity effusions. The intrathoracic and intraperitoneal organs are covered by a single layer of mesothelial cells, which is continuous with the lining of the thoracic and peritoneal cavities. The potential space between the two layers of epithelium contains a small amount of lubricating fluid

How valuable is ascitic cytology in the detection and

The malignant cells may continue to proliferate even after they are exfoliated into a serous cavity fluid to give rise to 'cell balls' known as 'proliferation spheres' (see Chapter 3, Figures 3.9, 3.10, 4.1, 4.3).These proliferation spheres are three-dimensional, solid or hollow aggregates without a stromal core (Figure 3.10).They are unique to metastatic cancer cells in serous cavity. A paracentesis was performed, revealing an ascitic fluid containing 1746 leucocytes/µL, of which 40% were eosinophils. Notably, the serum-ascites albumin gradient was <1.1 g/dL, the ascitic protein >3.5 g/dL, and cytology positive for malignant urothelial cells . Diagnostic evaluations for bacterial peritonitis, tuberculosis, parasitic.

JMalignant: Malignant Ascites DefinitionMesothelial cytopathology - Libre Pathology

Fluid Cytology Update: the Many Faces of Mesothelial Cells

predominant cells in pleural fluid were myeloma cells (Fig. 2). Intheother,amanof70yrwithknown myelomatosis and an encysted pleural effusion, myelomacells were found in small numbers among abundant red cells and other nucleated cells (inclu-ding a few myelocytes and normoblasts); but the diagnosis could not have been madeon cytological grounds cytology. Serum:Ascitic Albumin Gradient (SAAG) = serum albumin - ascitic fluid albumin. > 11g/L = high SAAG = transudate. < 11g/L = low SAAG = exudate. Cell count and differential. > 250 neutrophils/mm3 = spontaneous bacterial peritonitis. > 250 WCC = spontaneous bacterial peritonitis. polymorphonuclear cells - bacterial In the clinical routine, cytological analysis and biochemical tests are used to confirm peritoneal carcinomatosis or portal hypertension as primary cause for ascites . Malignant ascites caused by peritoneal carcinomatosis is associated with a poor outcome [8,9]. In the last decade, the understanding of the biology of malignant ascites has evolved Meanwhile, the cytology expertise of pathologists centrally checks for the presence of malignancy at a diagnostic-level rather than at a screening-level, yielding various sensitivities as reported; for example, an average of around 70% in detecting malignant cells in pleural fluid [3] and 40-65% in peritoneal fluid [4] In the present series, the pleural or ascitic fluid from eight patients showed malignant cells with characteristics of mesothelial cells. Four of these have been seen during the last four years, in three the diagnosis of mesothelioma was initially sug-gested onthe basis ofthe cytological findings. Features which enabled a mesothelial cell origi

Malignant Ascites: Diagnosis and Management - Cancer

The concentration of red blood cells is usually lower than 1000 cells/mm³ in ascitic fluid. Cytology examination of ascitic fluid can help diagnose malignant causes of ascites. Gram staining of ascitic fluid is usually not indicated as it is very rarely helpful. Ascitic fluid culture for mycobacteria has about 50% sensitivity of diagnosis Effusion cytology - Diagnosis. 1. NAMRATHA EFFUSION CYTOLOGY 2. Body cavities - Two layers derived from the embryonal mesenchyme- the visceral layer covers the organs while the parietal layer lines the outer wall. Together they form a self-contained cavity that is lined with mesothelial cells called a serous cavity; specifically, the pleural cavity surrounding the lungs, the pericardial cavity. Malignant ascites dr. varun 1. Malignant ascites (abnormal accumulation of fluid in the peritoneal cavity ) is a manifestation of end stage events in a variety of cancers and associated with significant morbidity. Itsonset and progression is associated with deterioration in quality of life (QoL) and a poor prognosis The positive cytology of the ascitic fluid for malignant cells is expected only in cases with peritoneal carcinoma. Buried ascites have high concentrations of triglycerides, usually higher than the serum. The triglycerides level should be obtained routinely in the presence of the asynchronous milk liquid Ascitic fluid cytology poses a problem in the diagnosis of rare malignant neoplasms such as yolk sac tumors because there is little literature describing the cytologic findings of yolk sac tumors in peritoneal fluid. Tumor cells resembling various carcinomas can also be difficult in determining the diagnosi

Fractal dimensions of reactive mesothelial cells and

In these patients, ascitic fluid cytology and fine-needle aspiration of adnexal mass are performed and a combination of cytomorphology and immunocytochemistry on cell blocks is required. High-grade serous carcinoma shows positivity for CK7, PAX8, WT1, and p53, enabling a specific diagnosis in these patients with advanced-stage disease prior to. Figure 1: (a1 and a2) The pleural fluid smears were very cellular comprising of three-dimensional papillaroid and tubular clusters of malignant cells. The tumor cells showed moderate pleomorphism, clumped chromatin, and prominent nucleoli. In addition, numerous mitotic figures were also noted (Papanicolaou stain)


The diagnostic yield of pleural fluid cytology ranges from 40% to 87% and that of ascitic fluid cytology ranges from 56.7% to 60%.[5,6,7,8] The parameters looked for in an effusion fluid sample are type of cells present, any abnormal increase in number of cells present and their arrangement, cell sizes, and its variability including cytoplasmic. Mesothelial cells in ascitic fluid, the associated tumor antigen 90K is known to possess properties similar cytokine in modulating the cellular immune system, where accessory cells are the main target of this molecule. In 67 patients with ovarian cancer with significant amounts of ascites, were immune-stimulating proteins 90K detected in all. Ascitic fuid cytoloyy is one of the frst tests conducted in a patient with ascites, both for confrmation of a suspected maliynancy and stayiny of a known maliynancy. Te detection of maliynant cells in ascitic fuid is carried out by the patholoyist by liyht microscopic examination Two cases of small-cell carcinoma of the ovary in the ascitic fluid and peritoneal/pelvic washings of a 30- and 28-yr-old woman, respectively, are presented and discussed. Smear preparations from the ascitic fluid showed loose clusters and single malignant cells with scant cytoplasm and nuclei with smooth to irregular nuclear membranes. Effusion cytology is the study of individual cells from aspirated material for the diseases diagnoses, accurate diagnosis of cells of serous fluids is a major challenge and distinguishing benign from malignant may require meticulous screening (1,2). Cytology gives the first indication of malignanc